BO2.7 - Practicals based on course BO2.4
Mangesh HajareSIRSHA MITRAArchana Naik

BO2.7 - Practicals based on course BO2.4

BO 2.7Practicals based on BO 2.4 (2C) 

Any 08 Practicals 

1. Estimation of soluble proteins in germinating and non-germinating seeds by Lowry/ Bradford’s method 2P 

2. Estimation of total amino acids in germinating and non- germinating seeds 1P 

3. Estimation of ascorbic acid in ripe and unripe fruits 1P 

4. Bioassay of Cytokinin concentration using test system of greening of cotyledons 2P 

5. Studies on induction of amylase activity by GA3 in germinating cereal grains 2P 

6. Measurement of respiration and photosynthetic rates using oxygen electrode (demonstration) 1P 

7. Measurement of CO2 uptake using IRGA (Demonstration) 1P 

8. Assay of Nitrate reductase activity 2P 

9. Assay of PEPcase activity in a C3 and C4 plants 2P 

10. Assay of invertase activity 1P 

11. Effect of substrate concentration and pH on enzyme activity. 2P 

12. Separation and measurement of flavonoids using chromatography 2P

 13. Separation and measurement of Anthocyanins from flowers of different stage of maturation. 1P

Plant Physiology and Biochemistry
Mangesh HajareSIRSHA MITRAArchana Naik

Plant Physiology and Biochemistry

BO 2.4 Plant Physiology&Biochemistry (3C)

Credit 1 (15L)

1.Structure and properties of water: Biological significance. Ionization of water,   pH, buffers   2L

2. Bioenergetics: Free energy, changes in free energy during chemical reactions, entropy and enthalpy,  

  high energy compounds, synthesis of ATP, activation   energy                                                   1L

3.  Enzymology: Classification and properties of enzymes, Isoenzymes, coenzymes    and cofactors,  

   coupled reactions, units of enzyme activity. Enzyme kinetics substrate concentration and rate;

   Competitive and noncompetitive inhibitors.  Covalent and allosteric regulation.                          3L

4. Building blocks of biological macromolecules: Biosynthesis and metabolism of amino acids,

    carbohydrates, fatty acids and lipids, purine and pyrimidine bases                                               3L

5. Mineral nutrition of plants: Cation-anion exchange capacity of soil, types of ion transporters,

    passive and active transport, primary and secondary active transport, Role of

    membrane potential in ion transport, high and low affinity transporters.                                       2L

6. Nitrogen: Uptake, assimilation and remobilization of nitrogen in plants, Biological  nitrogen

   fixation by free living and symbiotic organisms, mechanism of nitrogen fixation.                       3L

7. Phloem structure and function: Source and sink relationship, translocation of photoassimilates

   through phloem, phloem loading and unloading, composition of phloem sap.                               1L


Credit 2 (15L)

1.Water uptake, transport and transpiration, Stomatal physiology, mechanism and regulation of

   guard cell                                                                                                                                         2L

2. Photosynthesis– Photosynthetic pigments, organization of photosynthetic electron transport system    

   in thylakoid membranes. Charge separation and electron transport, fluorescence and  

   photochemistry, oxygen evolution, NAPD reduction,  photophosphorylation.                              4L

3. Reduction of carbon dioxide - RuBPcase and Calvin cycle, photorespiration. CO 2

    concentrating mechanisms in C4and CAM plants.                                                                         3L

4. Respiration – Glycolysis, citric acid cycle, pentose phosphate pathway. Organization of

    mitochondrial electron transport system, ATP synthesis. Respiratory control, anaerobic

   respiration                                                                                                                                        6L

Credit 3 (15L)

1. Plant growth hormones – Structure, biosynthesis and metabolism of auxins, cytokinins, gibberellins,    

   abscisic acid and ethylene. Physiological role of hormones                                                             8L

2. Photoperiodism and vernalization. Tropic and nastic movements in plants                                    3L

3. Secondary metabolites: Terpenoids, phenolics, alkaloids. Major secondary metabolite

  synthesis pathways in plants. Role of secondary m tabolites                                                           4L

BO2.2-Cell Biology

BO2.2-Cell Biology

This is 3 credit theory course created for the students of M.Sc.(Botany), Semester-II.